Since the advent of penicillin, antibiotics have become widely used as a treatment for most bacterial diseases. However, the increased use of them as a treatment has led the evolution of antibiotic resistance to proliferate across many species of bacteria, and the need for a greater variety of antibiotics has increased. This study stands to show the change in antibiotic resistance over the past 18 years, and see how mechanisms of action may affect this change. Data was collected by students who isolated and grew Staphylococcus in a lab setting. The bacteria was then plated and five different antibiotic discs were placed to measure antibiotic sensitivity. Overall, a change in resistance to antibiotics was not observed for any of the five antibiotics used, although resistance did fluctuate between each year. In the context of this study, the data may suggest that Staphylococcus has not been affected by antibiotic use, and continues to be sensitive to a variety of antibiotics. Yet, this contrasts what is expected and known about antibiotic resistance, especially with the identification of Methicillin-resistant Staphylococcus aureus (MRSA). As all data was student collected, it is difficult to control for confounding variables created by each student, and as such the results of this study should be regarded with great scrutiny.