Methodology
Mosquito population
The Anopheles albimanus Sanarate laboratory strain was used to isolate RNA and quantify boll and zpg orthologues gene expressions in each larvae stage and between sexes of pupae and adults. The mosquito strain is maintained in the insectary of Centro de Estudios en Salud (CES) of Universidad del Valle de Guatemala (Guatemala, Guatemala)  at 27-29 ÂșC and 70% relative humidity.
Primer design
Primers were made employing Oligo Analyzer version 3.1 (IDT).  Sequences of actin, zpg and  boll orthologe were retrieved from Vector Base (Table 1).