Results
Claudin-23 localizes to cell-cell junctions in Xenopus laevis epithelium
To confirm that Claudin-23 has the expected localization at cell-cell junctions in Xenopus laevis, eGFP-Claudin-23 and BFP-ZO-1, a TJ plaque protein, were coexpressed in oocytes by injection at 2-4 cell stage. They were then live imaged at gastrula stage. Claudin-23 colocalized with ZO-1 in an XY view, and a line scan across three junctions showed that the peak intensities of Claudin-23 and ZO-1 overlap (Figure 1).