Since 2013 the citi-bike has been operated in NYC and the whole dataset has been opened to the public. Analysis citi-bike trip duration behavior by gender by analysis the open data and plotting the two genders trip duration histogram. Null Hypothesis is average of the citi-bike women's trip duration is the same as the men's trip duration. Mann Whitney U test is used to test hypothesis test because the two histogram is non-gaussian distribution. Significant level is chosen to 0.05 and p-value of the hypothesis test is 0.0. Therefore, Null hypothesis can not reject it.