Week 0 | Streak and isolate L. lactis (wild type, and transformed), Make M17 media with 0.5% glucose. |
Week 1 | Test growth rates and abundance between the L. lactis wild type and transformed strain. Make M17 broth with 0.5% glucose. Grow L. lactis measuring the growth curve for four days, measuring every 12 hours using a spectrophotometer. |
Week 2 | Test growth rates of the L. lactis wild type and conjugated strain in the different pH, 5.7 and 7.3 by creating pH specific broth cultures. Growing the strains on a four day growth curve. Measuring growth every 12 hours using a spectrophotometer. |
Week 3 | Test growth rates of the L. lactis wild type and conjugated strain incubating in 40 degrees Celsius. Using a four day growth curve, measuring density every 12 hours. |
Week 4 | Determine the presence of plasmid through five generations of the L. lactis growth in M17 broth with 0.5% glucose. Plasmid presence will be confirmed by a plasmid extraction kit. |
Week 5 | We will insert the naked plasmid into M17 broth with 0.5% glucose of each of the following microbes: Salmonella enteridis, Yersinia enterocolitica, Campylobacter jejuni, B. subtilis, and E. coli. Plasmid presence will be determined by streaking the pathogens on M17 0.5% glucose plates with chloramphenicol. |
Week 6 | Colonies present on the chloramphenicol plates with growth will be run through a plasmid extraction kit to confirm plasmid presence, assuring growth was not a random mutation. |
Week 7 | Data analysis using Microsoft Excel. |