• Evaluate the possibilities of Gram-positive to Gram-negative gene transfer when using a plasmid containing antibiotic resistance.
Hypothesis:
a) We hypothesize that there will be persistent stability of the Nisin Inducible Expression system (NICE) vector plasmid inside the Lactococcus lactis and the species itselfover changes in pH, temperature, and growth pressure, similar to what is found in rumen.