Time line: (>1 page)
Hypothesis: 
    A. survival of the plasmid inside the Lactococcus lactis, via the NICE vector 
    A. Changes in pH, temperature, and growth pressure will not effect the stability of the NICE vector inside Lactococcos lactis.
B. Spreading of the plasmid and antibiotic resistance
C. will the naked plasmid be uptake through transformation by other pathogens, following lysis of host cell. 
Hints:
Week 0
 Make prep stuff: plates, broths, and frozen stalks, start growing plates and getting pure cultures to start (E. coli, B. subtilis, Salmonella, Typhe (typhoid)Camplyobacter, Yersinia enteraclidica ) 
Week 1
Plate the Lactococcus lactis and grow them over (x(get this from literature)) number of generations. This is going to extend beyond week one. 
Broth culture and check heat stability. See at what point the plasmid gets cured, ie when does resistance get lost. 
Growing some of the rumen strains with naked plasmid and testing rate of transformation ie see how much resistance is happening. 
Buy List: 
References and links: 
Frozen Stalk list 
  1. E. coli, Sept 21, 2017
  2. B. subtillus, Sept 21, 2017
  3.