Data Collection and Data Analysis
Timeline:
Week 0
Streak and isolate L. lactis (wild type, and transformed), Salmonella enteridis, Yersinia enterocolitica, and Campylobacter jejuni. Make M17 broth and media, make altered pH M17 broth and media
 
Week 1
Test growth rates and abundance between the L. lactis wild type and conjugated strain. Use M17 broth, dilute down and plate to determine starting titer.
 
Week 2
Test growth rates of the L. lactis wild type and conjugated strain in the different pH, 5.7, 6.5, 7.3. Measure using dilutions.
 
Week 3
Test growth rates of the L. lactis wild type and conjugated strain in the different temperature 38.7 and 40 degrees Celsius. Measure using dilutions
 
Week 4
Determine the presence of plasmid through three generations of the L. lactis in the most successful pH. Plasmid presence will be determined by PCR.
 
Week 5
Determine the presence of plasmid through three generations of the L. lactis grown at 38.7 degrees Celsius. Plasmid presence will be determined by PCR.
 
Week 6
We will insert the plasmid into TSB broth of each of the following microbes: Salmonella enteridis, Yersinia enterocolitica, Campylobacter jejuni, B. subtilis, and E. coli  and observe if the naked plasmid is absorbed by the pathogens.
 
Week 7
Plasmid presence in the enteric pathogens will the determined by running a PCR on each.
 
Week 8
Data analysis using Microsoft Excel.
 
Frozen Stalk list 
  1. E. coli, Sept 21, 2017
  2. B. subtillus, Sept 21, 2017
  3.    
Scope: Vaccination in ungulates both domesticated and wild (cattle and elk).