expected results: FAILURE, diminished growth and curing of plasmid (decreased efficacy) under different temperature and pH conditions. 
Diminished growth compared to wild type strains. 
  1. http://www.ansci.wisc.edu/jjp1/ansci_repro/lab/diseases_2002/brucellosis.html 
  2. “Facts about Brucellosis,” Aphis.usda.gov,  www.aphis.usda.gov/animal_health/animal_diseases/brucellosis/downloads/bruc- facts.pdf.
3.     J.E. Lowry, D.D. Isaak, J.A. Leonhardt, G. Vernati, J.C. Pate, G.P. Andrews, “ Vaccination with Brucella abortus Recombinant Induced Antigens reduces Bacterial Load and Promotes Clearance in a Mouse Model for Infection” PLoS ONE, 2011
5.      J. Neef, F.J. Milder, D.G.A.M. Koedijk, M. Klaassens, E.C. Heezius, J.A.G. van Strijp, A. Otto, D. Becher, J.M. van Dijl, G. Buist, “Versatile vector suit for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis,” Journal of Applied Genetics and Molecular Biotechnology, 2015(99)9037-9048.
6.  “M17 Agar.” CM0785, M17 Agar | Oxoid - Product Detail, www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=CM0785&org=82&.