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Implementation of Regional-CNN and SSD Machine Learning Object Detection Architectures for the Real Time Analysis of Blood Borne Pathogens in Dark Field Microscopy
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  • Daniel Fleury,
  • Febi Fleury
Daniel Fleury
John Marshall
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Febi Fleury
University of Minnesota


The emerging utilization of visualization techniques in pathology and microbiology has been accelerated by machine learning (ML) approaches towards image preprocessing, classification, and feature extraction in an increasingly complex series of datasets. Modern Convolutional Neural Network (CNN) architectures have developed into an umbrella of vast image reinforcement and recognition methods, including a combined classification-localization of single/multi-object featured images. As a subtype neural network, CNN creates a rapid order of complexity by initially detecting borderlines, edges, and colors in images for dataset construction, eventually capable in mapping intricate objects and conformities. This paper investigates the disparities between Tensorflow object detection APIs, exclusively, Single Shot Detector (SSD) Mobilenet V1 and the Faster RCNN Inception V2 model, to sample computational drawbacks in accuracy-precision vs. real-time visualization capabilities. The situation of rapid ML medical image analysis is theoretically framed in regions with limited access to pathology and disease prevention departments (e.g. 3rd world and impoverished countries). Darkfield microscopy datasets of an initial 62 XML-JPG annotated training files were processed under Malaria and Syphilis classes. Model training were halted as soon as loss values were regularized and converged.