3.3.8. Simulated small intestine duodenal digestion When gastric digestion was completed, the gastric digesta (214.5 ml) was released and added to the dynamic duodenal model at the entry of the lumen (Figure 3.1. describes the model). After addition, simulated segmentation and peristaltic motions were initiated, section 3.3.2., describes and discusses the operation of the model. From the secretory port, pancreatic mix solution (57ml), containing room temperature dissolved trypsin 0.126g from porcine pancreas-lyophilized powder, (~780 BAEE units/mg) from Applichem (A4148), α-Chymotrypsin 0.008g from bovine pancreas-lyophilized powder, 350U/mg (11.9 BTEE U/mg) and α-amylase 1.007g were added, along with HMS (20ml) and KRB (80ml). Prior to intestinal digestion the model luminal membrane was conditioned with 50 ml KRB.