Figure 1: Cohort definition and study design. A) Flow chart summarizing included samples, exclusion criteria, and clinical response. B) Study design, indicating the sample size in each group. Serum samples underwent screening using Next Generation Sequencing (miRNA) and proteomics screening, followed by a confirmation on a larger cohort using RT-qPCR and ELISA. Skin microbiota were quantified by RT-qPCR. C-D) Age and sex distributions in the studied cohort and their corresponding controls. E)Distribution of SCORAD values in studied AD patients cohort before initiation of therapy. F) Clinical parameters after dupilumab therapy in low, high, and super responders.