This paper describes a microwell-based microdevice for performing quantitative bioanalysis. This microdevice combined the passive pumping by degassed polydimethylsiloxane (PDMS) with serial operations including solution dispensing, plates splitting and plates stacking. We name this microdevice “stacking chip”. To use the stacking chip in quantitative bioanalysis, nanoliter solutions were first dispensed into the microwells through the degassed PDMS microchannels. Next, we split the microwell and microchannel plates assisted by the application of one drop of silicone oil, which resulted in a microwell array containing the reagent solutions. Microreactor arrays were formed by stacking the two microwell arrays containing the reagent solutions. With this microdevice, the enzymatic kinetics of alkaline phosphatase during the dissociation of the fluorescein diphosphate was measured and analyzed by the Michaelis-Menten model. The stacking chip is simple to fabricate and operate, and amenable to automation for high throughput analysis.

Introduction

The format of this template follows a typical journal publication with an introduction, results and conclusion. Examples of an equation, list and citation are also included. 

The purpose of the introduction

Most academic introductions follow an ‘inverted pyramid’ structure: they start broad and narrow down to a specific thesis or research question. The introduction should reveal:
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  2. references to related and prior work in the field of investigation
  3. succinct overview of the major point of the paper

Results

This section is only included in papers that rely on primary research. This section catalogues the results of the experiment. The results should be presented in a clear and unbiased way. Most results sections will contain links and citations, e.g., \cite{Feynman_1986}, and equations, e.g. \(e^{i\pi}+1=0\).

Conclusion

The conclusion should reinforce the major claims or interpretation in a way that is not mere summary. The writer should try to indicate the significance of the major claim/interpretation beyond the scope of the paper but within the parameters of the field. The writer might also present complications the study illustrates or suggest further research the study indicates is necessary.