\documentclass[10pt]{article}

\usepackage{fullpage}
\usepackage{setspace}
\usepackage{parskip}
\usepackage{titlesec}
\usepackage[section]{placeins}
\usepackage{xcolor}
\usepackage{breakcites}
\usepackage{lineno}
\usepackage{hyphenat}





\PassOptionsToPackage{hyphens}{url}
\usepackage[colorlinks = true,
            linkcolor = blue,
            urlcolor  = blue,
            citecolor = blue,
            anchorcolor = blue]{hyperref}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@combinedblfloats{\box\@outputbox}{\unvbox\@outputbox}{}{%
  \errmessage{\noexpand\@combinedblfloats could not be patched}%
}%
\makeatother


\usepackage[round]{natbib}
\let\cite\citep




\renewenvironment{abstract}
  {{\bfseries\noindent{\abstractname}\par\nobreak}\footnotesize}
  {\bigskip}

\titlespacing{\section}{0pt}{*3}{*1}
\titlespacing{\subsection}{0pt}{*2}{*0.5}
\titlespacing{\subsubsection}{0pt}{*1.5}{0pt}


\usepackage{authblk}


\usepackage{graphicx}
\usepackage[space]{grffile}
\usepackage{latexsym}
\usepackage{textcomp}
\usepackage{longtable}
\usepackage{tabulary}
\usepackage{booktabs,array,multirow}
\usepackage{amsfonts,amsmath,amssymb}
\providecommand\citet{\cite}
\providecommand\citep{\cite}
\providecommand\citealt{\cite}
% You can conditionalize code for latexml or normal latex using this.
\newif\iflatexml\latexmlfalse
\AtBeginDocument{\DeclareGraphicsExtensions{.pdf,.PDF,.eps,.EPS,.png,.PNG,.tif,.TIF,.jpg,.JPG,.jpeg,.JPEG}}

\usepackage[utf8]{inputenc}
\usepackage[english]{babel}










\begin{document}

\title{Unnamed Article}



\author[1]{Yaojuan}%
\affil[1]{Affiliation not available}%


\vspace{-1em}



  
  \date{May 05, 2024}


\begingroup
\let\center\flushleft
\let\endcenter\endflushleft
\maketitle
\endgroup








\sloppy


Establishment and transfer of classical eyeblink conditioning using
electrical microstimulation of the hippocampus as the conditioned
stimulus

% First macro on next line not (yet) supported by LaTeXML: 
% \protect\hypertarget{OLEux5fLINK11}{}Juan Yao\textsuperscript{1,2*},
Bing Wu\textsuperscript{1,2*}, Guang-yan Wu\textsuperscript{2}, Xuan
Li\textsuperscript{2}, Jian-ning Ye\textsuperscript{3\#}, Jian-feng
Sui\textsuperscript{1,2\#}

\textsuperscript{1}Department of Physiology, of Medical Science, ,
400038, PR China

of Basic Medicine, Medical Science, Third Military Medical

University, 400038, PR China

\textsuperscript{3}Department of Neurology, , , 400038, PR China

Abstract:

The present experiment was designed to determine whether classical
eyeblink conditioning (EBC) can be established under the conditions of
using electrical microstimulation of hippocampus as the conditioned
stimulus (CS) paired with airpuff unconditioned stimulus (US), to
observe whether EBC transfer would occur when CSs shift between
microstimulation of hippocampus as a CS (Hip-CS) and tone as a CS (tone
CS), and to compare the difference in transfer effectiveness between
delay EBC (dEBC) and trace EBC (tEBC). A total of 8 groups of guinea
pigs, including 4 experimental groups and 4 control groups were included
in the study. The experimental groups accepted Hip-CS (or tone CS)
paired with US firstly; then, shifted CS to tone CS (or Hip-CS) and
paired with US. The control groups accepted corresponding Hip-CS (or
tone CS) and pseudo-paired with US, and then shifted to tone CS (or
Hip-CS) and paired with US. Results show that EBC could be successfully
established when using microstimulation of hippocampus as the CS pairing
with airpuff US, and that acquisition rates of EBC were higher in
experimental groups than in control groups after switching from Hip-CS
to tone CS or vice versa, indicating the occurrence of learning transfer
between EBC established with Hip-CS and tone CS. The present study also
demonstrated that EBC acquisition rates were remarkably higher in dEBC
than in tEBC in both transfer ways, suggesting that memory of CS--US
association was less disturbed in dEBC than in tEBC by the sudden switch
of CSs. The result is of significance to expand our knowledge about EBC
transfer as well as the functional neural circuit underlying EBC
transfer.

\selectlanguage{english}
\FloatBarrier
\end{document}

