Figure 2: A. SDS-PAGE (Coomassie stained) analysis of
process efficiency, extraction at pH 4: Lane 1: Total hIgG added to the
system; lane 2: E. coli. lysate added as an artificial
contamination background; lane 3: E. coli lysate and total hIgG;
lanes 4-9: hIgG recovered via conjugated micellar aggregates comprising:
Brij-O20, the
[(bathophenanthroline)3:Fe2+]
complex and amino acid monomers (see the Materials and Methods section);
lane 10: Molecular weight markers (KDa). Red arrows indicate reduced
heavy and light chains of hIgG, respectively. B. Process yield
when human IgG (input concentration, 5 mg/ml), is extracted at pH 4,
with addition of indicated amino acid monomers, while the Control lacks
amino acid monomers; C. As in B , but with extraction
at pH 6.3. Error bars are calculated as the standard deviation of 4
replicate measurements conducted on different days. Quantitation was
accomplished as described in the Materials and Methods section.