Dynamic light scattering (DLS)
DLS measurements were performed by comparing commercial, pure
(>95%) hIgG (1-2 mg/mL) that had not encountered
detergent, with hIgG following capture and extraction viaconjugated Brij-O20 micelles ± amino acid monomers. All hIgG samples
were solubilized in 200 mM Tris pH 6.3 and 50 mM NaCl. Samples were
centrifuged at 21,000 x g for 20 minutes at at 19 °C prior to
measurement at 25oC. The intensity-weighted size
distributions of hIgG were determined using the auto-correlation
spectroscopy protocol of the Nanophox instrument (Sympatec GmbH,
Clausthal-Zellerfeld, Germany).