Cefazolin is a broad-spectrum first-generation cephalosporin antibiotic, used for the treatment of a number of bacterial infections. The aim of this research work was to evaluate the influence of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical, and thermal properties of cefazolin sodium powder using modern analytical techniques. The test sample cefazolin sodium was divided into two parts. One part of the test sample was considered as a control sample (no Biofield Energy Treatment was provided), whereas the other part was treated with the Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as a treated sample. The particle size values in the treated sample were significantly decreased by 3.95%(d10), 2.63%(d50), 13.02%(d90), and 21.7% {D (4,3)}; hence, the specific surface area was increased by 5% compared to the control sample. The decomposition temperature of the treated cefazolin was increased by 4.84 compared to the control sample. Similarly, the latent heat of evaporation of two peaks and decomposition was significantly increased by 56.81%, 225.38%, and 261.84% in the treated sample compared with the control sample. The total weight loss was decreased by 2.7%; however, the residual amount was significantly increased by 28.59% in the treated cefazolin compared with the control sample. A new form of cefazolin sodium might have generated after Trivedi Effect®-Consciousness Energy Healing Treatment which may offer better solubility, dissolution rate, bioavailability, and thermal stability compared to the control sample. The treated cefazolin sodium would be more efficacious pharmaceutical formulations against respiratory tract infections, cellulitis, urinary tract infections, joint infection, biliary tract infections, pneumonia, endocarditis, genital infections, blood infections, etc