ABSTRACT
This paper describes a new method for preparation of chromogen free
oxidase based optical biosensors. Oxidases enzymes were deposited on the
titania film consisting of uniformly distributed nanoparticles reacting
with hydrogen peroxide thus providing formation of yellow color due to
complex Ti (IV)-H2O2. Using ink jet
printing and specially developed titania ink we have created a universal
platform for the oxidase based biosensors and successfully tested it on
glucose and cholesterol detection. The paper fully discusses new
biomaterials as well as biosensor creation technology and describes the
mechanism of action, study on stability and working concentration limits
and adjusting composition of the inks for good printability and scale-up
production.