Figure 3. Conception of the biosensor production from sensor preparation to working principle
We employed wax-printing technology to create the color scale which can be used for visual evaluation of measurement result, color-corrected and calibrated by X-Rite eXact device (Figure 4 a,b) on the biosensor surface.
Thus, we prepared 50 samples of titania substrates and checked their sensitivity to hydrogen peroxide, and the reproducibility of the analysis. As you can see in Figure 4c, under optimum printing conditions it is possible to achieve high repeatability of thickness and stability of optical response. We applied the same amount of hydrogen peroxide with all 50 samples and then the optical response was recorded. The experiment shows that our average standard deviation does not 0.07 in relative intensity, which is a valid indicator of repeatability.