Production of biosensor
For biosensor production we have used matte photo paper Lomond, gsm 230,
which was subjected to several steps of printing applying few layers.
Firstly, we printed on photo paper a specially prepared template with
the boundary lines for the production of test strips and color chart for
the comparison with the test area. The coating was applied to both sides
of the paper by Xerox Phaser 8860 printer with solid wax ink. Thus, we
created a hydrophobic coating on paper and the small limited area of a
sensing element. Then using this pre-defined template with hydrophobic
coating, we applied titania ink into the sensing zone by inkjet printer.
For this purpose we used the titania ink, which was prepared by the
method described above. The ink was placed in Dimatix 10 pl cartridge
and using the template printing was carried out only in the pattern
areas where the hydrophobic coating was not applied. Thereby the
positioning of the sensitive area was strictly on the paper. After
printing, the sample was kept to dry completely. Finally, after drying
the biosensor preform the recognizing element containing specific
oxidases was placed on titania layer by inkjet printer. Dimatix 10 pl
cartridge was filled with bio-ink based on enzyme solutions made by the
method described above. The printing was performed with the use of the
same template and the ink was applied only on titania areas without
hydrophobic coating. After the printing the biosensor was kept under
vacuum to dry and assayed for H2O2,
glucose and cholesterol control solutions of different concentrations.