to image sample from a specific well-defined angle
Inhomogeneous samples have an optimal orientation when
studied by light microscopy. For example in developmental biology highly
scattering tissues such as pigments, the eyes or the yolk can obscure the organ
or tissue of interest. Typically, the
sample is carefully embedded in its ideal orientation prior to the experiment. However, after embedding
the sample’s orientation is fixed and cannot be adapted to the sample’s
development during in vivo experiments or to another region of interest anymore.
Define explicitly what the goal is
(with respect to your sample, zebrafish).
To study organisms with optimal
resolution during development, a technique to dynamically orient the sample in the microscope in any arbitrary
orientation is needed. (While different
organisms may require tailored solutions), the popular model organism zebrafish is
transparent in the early stages making it the ideal sample for in vivo imaging.
Embedding the work in the literature
To align many zebrafish larvae for high-throughput applications in the preferred orientation microfluidic systems have been developed Lin 2015
. For ... neuro zebrafish larvae have been embedded between two coverslips and imaged from two sides by manually turning
the sample Ronneberger 2012
. While these techniques allow a precise, static sample
orientation they still did not offer an adaptive
reorientation of the sample. In light-sheet microscopy (or SPIM