Protein from camelina seed is a valuable co-product that can be derived from the meal remaining after oil extraction. The current study describes the types and physicochemical properties of the major proteins present in camelina meal. Seed coat mucilage, which interferes with protein extraction, was removed from whole seeds by digestion with Viscozyme® and lipids were removed with hexane to obtain demucilaged/defatted meal. Protein comprised 51.3% of meal dry matter and the eight essential amino acids comprised 40.8% of total amino acids. The meal polypeptide profile showed bands originating from cruciferin (~44.1 and 51.7 kDa), napin (~14 kDa) and oil body proteins (OBP; ~15-20 kDa) resembling that of other crucifers. Cruciferins (11 isoforms) were the predominant proteins, while vicilins (6 isoforms) also were identified among the proteins soluble at pH 8.5. Among the proteins soluble at pH 3, napins (5 isoforms) comprised the majority, though late embryogenesis abundant proteins also were found. Camelina cruciferin and napin were confirmed to possess predominantly β-sheet and α-helix secondary structures, respectively. Camelina cruciferin structure was highly sensitive to changes in medium pH and underwent acid-induced denaturation at pH 3, but exhibited high thermal stability (>80°C) at neutral and alkaline pHs. The structure of camelina napins was less sensitive to pH. The major proteins associated with oil bodies were oleosins (6 isoforms). Identification and characterization of the properties of camelina meal proteins will enable strategic paths for co-product valorization.
Objectives Endoscopic endonasal transsphenoidal adenomectomy (TSA) is the most frequently performed skull base surgery and researchers have recently focused on preserving nasal function. The transseptal approach is a promising procedure owing to its less injury to nasal mucosa. Therefore, we conducted this study to investigate whether the transseptal approach could reduce nasal morbidities with comparable neurosurgical outcomes. Study design Retrospective chart review. Setting Single-institution tertiary care centre. Participants 25 patients of transseptal approach from January 2019 to December 2020 and 25 patients of transnasal approach from January 2017 to December 2018. Main outcome measures Lund-Kennedy endoscopic scores, 22-item sinonasal outcome test (SNOT-22) scores, TIB smell identification tests (TIBSIT®) scores, the rate of gross total resection, endocrine control, and postoperative complications were collected. Results The postoperative SNOT-22 (4.40 vs. 7.36, P =.005) and Lund-Kennedy endoscopic scores (2.72 vs, 5.08 at 2 weeks, 0.88 vs. 3.52 at 1 month, 0.48 vs. 2.12 at 3 months, 0.04 vs. 1.76 at 6 months, all adjusted P < .01) were significantly lower in the transseptal group, while the TIBSIT scores were not significantly different (41.6 vs. 42.4 preoperatively, P = .68; 41.6 vs. 40.2 postoperatively, P = .46). The rates of gross total resection, recovery of hormonal abnormalities, and complications were not significantly different. In multivariate analysis, the transseptal approach remained an independent factor for lower SNOT-22 scores and Lund-Kennedy endoscopic scores. Conclusion The transseptal approach provides better recovery of nasal mucosa and intact olfactory function without compromising neurosurgical outcomes.
This report describes the case of an 84-year-old male who was brought to the emergency room because a dental bur was swallowed accidentally during a dental procedure. The foreign body was successfully removed by gastroenterologists endoscopically 8 days after the ingestion and was identified as a 2-cm-long dental bur.
Neuroendocrine tumors have a wide range of malignant potential. These tumors infrequently metastasize to the orbit. We report the case of a 61-year-old man who presented with unilateral recurrent panuveitis related to choroidal metastasis. Explorations led to the diagnosis of pancreatic neuroendocrine carcinoma as the primary tumor.
A 77-year-old female patient was taken to surgery for correction of complete rectal prolapse. On the first postoperative day the patient presented severe generalized abdominal pain, with no other symptoms. l. An abdominal CT scan was performed, which showed pneumoperitoneum and air near the coloanal anastomosis
Background: Dysregulation of the arachidonic acid metabolic pathway is the most widely known pathomechanism of AERD. We performed integrative analysis of transcriptomic and epigenomic profiling with network analysis to determine the novel pathogenic features of AERD. Methods: Ten patients with asthma including 5 patients with AERD and another 5 patients with aspirin tolerant asthma (ATA) were enrolled. Nasal epithelial scraping was performed and nasal mucosa was used in omics profiling. Peripheral eosinophil counts, sputum eosinophil counts, FeNO levels, and pulmonary function test results were evaluated. Differentially expressed genes (DEGs), differentially methylated probes (DMPs) and differentially correlated genes (DCGs) between patients with AERD and those with ATA were analyzed. Network analysis using Ingenuity Pathway Analysis (IPA) was performed to determine the gene connection network and signaling pathways. Results: In total, 1,736 DEGs and 1,401 DMPs were identified. Finally, 19 pairs for DCGs were selected. Among DCGs, genes related to vesicle transport (e.g. STX2 and RAB3B) and sphingolipid dysregulation (e.g. SMPD3) were found to be hypo-methylated and up-regulated in patients with AERD. A total number of 78 asthma-related DEGs were identified by the IPA knowledge base. Using the canonical pathway analysis of IPA, signaling pathways of T helper cell differentiation/activation and Fcε receptor I were generated. Up-regulation of RORγt and down-regulation of MHCII, TNFR, and TGF-β as well as up-regulation of FCER1A and JAK and down-regulation of VAV and cPLA2 were noted in patients with AERD. Conclusions: Distinct pathogenic features were identified by using integrative multi-omics data analysis in patients with AERD.
Introduction: Point of care ultrasound (POCUS) is a useful tool to determine endotracheal tube placement; however, few studies have compared it with standard methods of confirmation. We evaluated the diagnostic accuracy of POCUS and time-to-interpretation for correct identification of tracheal versus esophageal intubations compared to a composite of standard-of-care methods in neonates. Methods: A cross-sectional study was conducted in the Neonatal Intensive Care Unit (NICU) at Aga Khan University Hospital Karachi Pakistan. All required intubations were performed as per NICU guidelines. The ETT placement was determined using standard-of-care methods (auscultation, colorimetric capnography, and chest X-ray) by a clinical team, and simultaneously by POCUS. Timings were recorded for each method by independent study staff. Results: A total of 348 neonates were enrolled in the study. More than half (58%) of intubations were in an emergency scenario. Using an expert as the reference standard, POCUS user interpretation showed 100% sensitivity and 94% specificity. We found a 99.4% agreement (Kappa: 0.96; p<0.001) between the POCUS user and expert. Diagnostic accuracy of POCUS compared with at least two standard-of-care methods demonstrated 99.7% sensitivity, 91% specificity, and 98.9% agreement (Kappa:0.93; p<0.001). The median time required for POCUS interpretation was 3.0 (IQR 3.0 -4.0) seconds for tracheal intubation. The time recorded for auscultation and capnography was 6.0 (IQR 5.0 -7.0) and 3.0 (IQR 3.0-4.0) respectively. Conclusion: POCUS is a rapid and reliable method of identifying ETT placement in neonates. Early and correct identification of airway management is critical to save lives and prevent mortality and morbidity.
Introduction: The reliability of breath sound analysis using an improved method in infants has been reported. Objective: Breath sounds of infants with respiratory syncytial virus (RSV)-induced acute bronchiolitis were analyzed to evaluate their specificity and examine their relationship with the severity. Subjects and methods: We evaluated the inspiratory and expiratory breath sound parameters of 33 infants (median age=2 months old) diagnosed with RSV-induced acute bronchiolitis. The sound powers of inspiration and expiration and the spectrum curve indices (ratio of the third and fourth area to the total area under the curve and ratio of the power and frequency at 50% and 75% of the highest frequency were evaluated at the acute phase and recovery phase. Furthermore, the relationship between the breath sound parameters and the clinical severity of acute bronchiolitis was examined. Results: Analyses of the breath sound spectrogram showed that the power of expiration at the acute phase was large, and was the expiration-to-inspiration sound power ratio in a mid-frequency range (E/I MF), with values decreased in the recovery phase. Data of the inspiratory sound spectrum curve showed the improvement of airway stenosis during the recovery period. Furthermore, there were significant relationships between the E/I MF and SpO2 and the severity score of acute bronchiolitis. Conclusion: In RSV-induced acute bronchiolitis, specificity of breath sounds was observed in the expiration sound and the reversible airway stenosis was evaluated. Breath sound analyses are expected to be useful for the diagnosis and clinical evaluation of the severity of acute bronchiolitis.
Environmental considerations are among the main reasons for selecting fuel cells as a strategic approach. To conduct a feasibility study for the application of a combined system in residential buildings for thermal energy (heat) and electricity (power) production, this paper comprehensively investigated the use of polymer fuel cells and solar energy in Farabi Administrative-Educational Building in the Islamic Azad University, Hamedan Branch, Iran. This educational building is located in the cold arid climate of Hamadan, which is a high-altitude city, and its working hours coincide with solar radiation hours. The use of electricity produced by photovoltaic (PV) panels for hydrogen generation is a new effort to use solar energy in classrooms. Attempts were made to produce the fuel used in polymer fuel cells, i.e., hydrogen, by a polymer electrolyzer and solar energy with the objective of supplying electricity to Farabi Building with the highest cost-effectiveness. Part of thermal energy is supplied by heat removed from the fuel cell. The process was analyzed with the help of TRNSYS energy processor. According to the results, 4923 kW electricity is obtained by installing the designed system, reducing carbon dioxide production by 5662200 g on average.
The paper overviews and summarizes the results of the developing a methodology for the land degradation neutrality (LDN) assessment basing on LDN-based studies at national, regional and local levels in Russia. The review of more one hundred available publications in Russian language over the past 6-7 years allowed for analysis on the following areas: development of LDN terminology, LDN assessment at the different levels, adapting transition matrix; application of global and national LDN indicators; using the LDN concept for economic valuation of land, estimating LDN baseline, and using LDN as an integral indicator for sustainable land management. With the LDN concept the issue of land degradation (LD) has gone beyond the limited scope of desertification and drylands, and enlarged the concept of “rational” or “effective” land use and land management dominated in Russia. The LDN concept has been broadened with the introduction of the LDN Index proposed to evaluate the rate of LDN achievement; proposal on reconstructing transition matrices and adding specific land cover sub-categories; approach of integrating traditional national sectoral systems for assessing land quality with an LDN add-on; justification for using additional and specific LDN indicators at national and subnational level (soil erosion, aridity, soil salinity, soil depletion, etc.); importance of factoring natural background trends like climate change, natural succession cycles linked with geological and geomorphological processes; need for using different site-specific LDN baselines, not only those time-based; approaches for LDN-based economic valuation of lands; and the LDN-based typology of SLM practices and models.
Right atrial appendage aneurysms (RAAAs) are extremely rare in cardiac anomaly. According to the literature, no more than 25 cases have been reported so far, among which only 3 cases were children. Here, we reported an infant with a giant RAAA and severe symptoms. The RAAA was diagnosed by echocardiography and surgically resected under cardiopulmonary bypass. The role of transesophageal echocardiography was very important during the aneurysm resection surgery, which helped surgeons to plan surgical procedures during the surgery and evaluate the surgical effect postoperatively.
In this paper, we consider the equations of motion of a bar, of a given density, infinite in both direction, undergoing longitudinal vibrations under the action of an external load, and a stress-strain relationship using a fractional order differentiation operator with respect to the time variable. We use three types of fractional operators, two non-singular, one with exponential kernel and Mittag-Leffler type kernel. And a singular one with power-law type kernel. We analyze some initial and boundary value problems resulting from the modeling of the aforementioned wave processes in viscoelastic media, using some recent proposals for fractional order derivatives. The fundamental solution of these problems is established, and its moments are calculated.
In this study we consider the inverse problem of determining the nonlinear right-hand side of a quasi-linear parabolic equation and prove a uniqueness theorem. Stability estimate for the solution are obtained. A method of representing the nonlinear right-hand side explicitly is proposed for the special case.
The internal structure of packed beds formed by different arrangements of packings has been a subject of study for the past several decades. In this study, we have attempted to investigate the structure formed in laboratory-scale packed beds through experimentation and modeling, employing different ways of packing the bed. The structure has been measured using Micro-Computed Tomography (Micro-CT) and characterized through appropriate metrics. The “filling of the bed” has been modeled using a technique called Collision Guided Packing (CGP). Thus, this contribution combines both high resolution measurements as well as detailed particle-based models, bringing out a head-to-head comparison of the bed filling process and also how the final bed is configured (its structure). The work stands as an independent piece, but also provides the basis for a rigorous understanding of the impact of bed filling on deep hydroprocessing, wherein the bed structure is known to have a dramatic effect.
We studied the thermomechanical and microstructural properties of oleogels developed with 2.1 to 15.7 Moles of monoglycerides/Mole of lecithin (MG/LC). The oleogels were developed (15°C) in vegetable (VO) and mineral (MO) oils using at each MG/LC 2% or 4% total mass of gelator. During oleogelation a synergistic MG-LC interaction existed deriving in the development of MG-LC cocrystals even below the gelators’ minimum gelling concentration. The cocrystals delayed the Lα→β polymorphic transition and worked as an active filler of the oleogels’ crystal network. In the VO, the oil with the highest relative polarity, the oleogels were structured by a network of β crystals where the cocrystals acted as an active filler. In the MO, the oil with the lowest relative polarity, the cocrystals’ development was favored while the Lα→β transition occurred just in the 15.7 MG/LC oleogels. Then, at all MG/LC the VO oleogels with 2% or 4% total gelator concentration achieved higher G’ than MO oleogels. However, the presence of β crystals will produce deleterious effects in shorter time in the VO oleogels than in the MO oleogels. In both oils the oleogels with the highest G’ and gel-like rheological behavior were achieved at 8.1 MG/LC, particularly at 4% total gelator concentration. Under these conditions the β polymorph was limited developed in the VO oleogels and completely absent in the MO oleogels. Then, we might tailoring the rheology of MG-LC oleogels with storage stability using as design variables the MG/LC, the total gelator concentration, and the polarity of the oil.
Sensitization to shrimp and house dust mites in a Swedish population-based study: Influence on allergic disorders and lung functionIda Waern1, Magnus Molin2, Robert Movérare2,3, Jonas Lidholm2, Andrei Malinovschi4, Magnus P. Borres2,5, Christer Janson3.1Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden2Thermo Fisher Scientific, Uppsala, Sweden3Department of Medical Sciences: Respiratory, Allergy and Sleep Research, Uppsala University, Uppsala, Sweden4Department of Medical Sciences: Clinical Physiology, Uppsala University, Uppsala, Sweden5Department of Maternal and Child Health, Uppsala University, Uppsala, Sweden