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Single-cell analysis reveals microbial spore responses to sodium hypochlorite
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  • Weiming Yang,
  • Yufeng Yuan,
  • Lin He,
  • Haihua Fan
Weiming Yang
Dongguan University of Technology
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Yufeng Yuan
Dongguan University of Technology
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Lin He
Dongguan University of Technology

Corresponding Author:[email protected]

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Haihua Fan
South China Normal University School of Information and Optoelectronic Science and Engineering
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Pollution from toxic spores has caused us a lot of problems, because spores are extremely resistant and can survive most disinfectants. Therefore, the detection of spore response to disinfectant is of great significance for the development of effective decontamination strategies. In this work, we investigated the effect of 0.5% sodium hypochlorite on the molecular and morphological properties of single spores of Bacillus subtilis using single-cell techniques. Laser tweezers Raman spectroscopy showed that sodium hypochlorite resulted in Ca 2+-dipicolinic acid release and nucleic acid denaturation. Atomic force microscopy showed that the surface of treated spores changed from rough to smooth, protein shells were degraded at 10 min, and the permeability barrier was destroyed at 15 min. The spore volume decreased gradually over time. Live-cell imaging showed that the germination and growth rates decreased with increasing treatment time. These results provide new insight into the response of spores to sodium hypochlorite.
Submitted to Journal of Biophotonics
18 Feb 2024Review(s) Completed, Editorial Evaluation Pending
11 Mar 20241st Revision Received
11 Mar 2024Assigned to Editor
11 Mar 2024Submission Checks Completed
11 Mar 2024Reviewer(s) Assigned
28 Mar 2024Review(s) Completed, Editorial Evaluation Pending
28 Mar 2024Editorial Decision: Revise Minor
02 Apr 2024Editorial Decision: Accept