loading page

Cell viability and cytotoxicity assays: Biochemical elements and cellular compartments
  • +1
  • Khalef LEFSIH,
  • Lydia RADJA,
  • Filicia KHETTAR,
  • Moussa BERKOUD
Universite Mouloud Mammeri de Tizi Ouzou

Corresponding Author:[email protected]

Author Profile
Universite Mouloud Mammeri de Tizi Ouzou
Author Profile
Universite Mouloud Mammeri de Tizi Ouzou
Author Profile
Universite Mouloud Mammeri de Tizi Ouzou
Author Profile


Cell viability and cytotoxicity assays play a crucial role in drug screening and evaluating the cytotoxic effects of various chemicals. The quantification of cell viability and proliferation serves as the cornerstone for numerous in vitro assays that assess cellular responses to external factors. In the last decade, several studies have developed guidelines for defining and interpreting cell viability and cytotoxicity based on morphological, biochemical, and functional perspectives. As this domain continues to experience ongoing growth, revealing new mechanisms orchestrating diverse cell cytotoxicity pathways, we suggest a revised classification for multiple assays employed in evaluating cell viability and cell death. This classification is rooted in the cellular compartment and/or biochemical element involved, with a specific focus on mechanistic and essential aspects of the process. The assays are founded on diverse cell functions, encompassing metabolic activity, enzyme activity, cell membrane permeability and integrity, ATP content, cell adherence, reduction equivalents, dye inclusion or exclusion, constitutive protease activity, colony formation, DNA fragmentation and nuclear splitting. These assays present straightforward, reliable, sensitive, reproducible, cost-effective, and high-throughput approaches for appraising the effects of newly formulated chemotherapeutic biomolecules on the cell survival during the drug development process.
Submitted to Cell Biochemistry & Function
01 Feb 2024Review(s) Completed, Editorial Evaluation Pending
19 Feb 2024Editorial Decision: Revise Major
04 Mar 2024Review(s) Completed, Editorial Evaluation Pending
04 Mar 2024Assigned to Editor
04 Mar 2024Submission Checks Completed
04 Mar 2024Reviewer(s) Assigned