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OsACL-A2 is involved in iron transport and positively regulates iron dependent disease resistance
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  • Wenjing Duan,
  • Aaron Chan,
  • Peng Xu,
  • Yingxin Zhang,
  • Lianping Sun,
  • Beifang Wang,
  • Yongrun Cao,
  • Yue Zhang,
  • Min Jiang,
  • Dian Li,
  • Daibo Chen,
  • Yongbo Hong,
  • Xiaodeng Zhan,
  • weixun wu,
  • Shihua Cheng,
  • Qunen Liu,
  • Liyong Cao
Wenjing Duan
China National Rice Research Institute
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Aaron Chan
China National Rice Research Institute
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Peng Xu
China National Rice Research Institute
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Yingxin Zhang
China National Rice Research Institute
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Lianping Sun
China National Rice Research Institute
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Beifang Wang
China National Rice Research Institute
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Yongrun Cao
China National Rice Research Institute
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Yue Zhang
China National Rice Research Institute
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Min Jiang
China National Rice Research Institute
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Dian Li
China National Rice Research Institute
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Daibo Chen
China National Rice Research Institute
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Yongbo Hong
China National Rice Research Institute
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Xiaodeng Zhan
China National Rice Research Institute
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weixun wu
China National Rice Research Institute
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Shihua Cheng
China National Rice Research Institute
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Qunen Liu
China National Rice Research Institute
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Liyong Cao
China National Rice Research Institute

Corresponding Author:[email protected]

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Abstract

Iron is an essential nutrient required for growth, development and also impacts disease resistance in plants. Plant absorb iron through their roots and citrate plays a vital role in transport of Fe 3+ through the xylem. In this study, we found that the rice cytoplasmic ATP-citrate lyase subunit A2, OsACL-A2 ( Os12g0566300) serves as a crucial factor in iron uptake and translocation. Leaf iron levels were depressed in the osacl-a2 mutant which caused iron deficiency induced chlorosis, activated defense signaling and eventually necrosis in mature leaves. Furthermore, loss of OsACL-A2 activity reduced disease resistance in leaves that had not reached development of necrosis. Citrate levels are increased in the osacl-a2 mutant, while ACL activity in the wild type is upregulated in response to both iron starvation and pathogen attack. We hypothesize that OsACL-A2 activity generates source-sink gradients through targeted lysis activity thus directing the movement of citrate-Fe 3+.