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Isolation and antimicrobial profile of staphylococcus aureus from dairy cows in the two selected farm of Bishoftu town, Ethiopia
  • Felmata Kenei,
  • Nezif Zenu
Felmata Kenei
Wollega University

Corresponding Author:[email protected]

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Nezif Zenu
Wollega University
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Abstract

Staphylococcus aureus is a common causative agent of bovine mastitis in dairy herds worldwide causing huge economic losses to the dairy industry. A cross-sectional study was conducted from July 2021 to September 2021, with the objective of isolating and identifing of S­.aureus from dairy farms in the milk samples of Bishoftu town in the Oromia region, Ethiopia. The samples were transported to a microbiology laboratory where, isolation and identification of an organism was based on morphological, cultural and biochemical characteristics, Clinical examinations, gram stain, catalase test, oxidase test and bacteriological culture methods were used as diagnostic tools to carry out the study. Accordingly, a total of 53 milk samples of where checked, 43 from teat and 10 samples from milk tankers. Milk samples were collected and cultured in parallel on both blood and nutrient agars. Of the total samples, 30/53 was well grown on both media and sub_ cultured on nutrient agar for further identification using primary and secondary biochemical tests. Accordingly, 30 milk samples showed typical large, round, golden yellow colonies of Staphylococcus aureus that frequently surrounded by zones of clear beta-hemolysis when grown on blood agar, round shaped grape like clusters we observed under a microscope after staining with gram stain, catalase­_ positive Staphylococcus aureus with bubble formation was observed and typical yellow pigmentation was observed on mannitol salt agar which is a selective medium for the genus Staphylococcus. Finally, 30(56.6 %) case of Staphylococcus aureus were identified with the coagulase test and a clumping result observed. Hence, implementing hygienic conditions, creating awareness of control and prevention of subclinical mastitis in dairy farms and conducting drug sensitivity tests for Staphylococcus aureus are recommended.