loading page

Identification and Bioactivity Evaluation of Twelve New Dep-sidone Derivatives from Garcinia Oligantha
  • +5
  • Xiaohui Peng,
  • Sha Chen,
  • Xiaofan Liu,
  • Jinyuan Yang,
  • Fanzhu Meng,
  • Hao Cao,
  • Dahong Li,
  • Huiming Hua
Xiaohui Peng
Shenyang Pharmaceutical University
Author Profile
Sha Chen
Shenyang Pharmaceutical University
Author Profile
Xiaofan Liu
Shenyang Pharmaceutical University
Author Profile
Jinyuan Yang
Shenyang Pharmaceutical University
Author Profile
Fanzhu Meng
Shenyang Pharmaceutical University
Author Profile
Hao Cao
Shenyang Pharmaceutical University
Author Profile
Dahong Li
Shenyang Pharmaceutical University
Author Profile
Huiming Hua
Shenyang Pharmaceutical University

Corresponding Author:[email protected]

Author Profile

Abstract

Phytochemical studies on the leaves and twigs of Garcinia oligantha led to the isolation of twelve novel depsidone derivatives (oli-ganthdepsidones A-L, 1-12). Their structures were elucidated by extensive spectroscopic analysis including 1H and 13C NMR, HSQC, HMBC and NOESY along with HRESIMS. The structure of oliganthdepsidone J was finally determined using DFT-NMR chemical shift calculations and DP4+ methods. Cytotoxicity test in four human cancer cell lines indicated that oliganthdepsidone F (6) had relatively strong cytotoxic effect against A375 (melanoma), A549 (lung cancer), HepG2 (liver cancer), and MCF-7 (breast cancer) cell lines with IC50 of 18.71, 15.44, 10.92, and 15.90 μM, respectively. The dose- and time-dependent antiproliferative effects of oliganthdepsidone F on these cell lines were also observed by CCK-8 test. As determined by fluorescent microscopy and flow cytometry in these cell lines, oliganthdepsidone F could promote cell apoptosis, leading to the inhibition of cell proliferation. The results of wound healing assay and transwell assay showed that oliganthdepsidone F could inhibit the migration and invasion of A549 and MCF-7 cell lines in a concentra-tion-dependent manner.