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MiR-205-3p Modulates Hepatocellular Carcinoma-Derived Endothelial Cell Progression through suppression of HINT1
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  • Mengnan Wang,
  • qiqi mao,
  • Jianqiang Xiang,
  • Hong Li
Mengnan Wang
Ningbo Medical Centre Lihuili Hospital
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qiqi mao
Ningbo Medical Centre Lihuili Hospital
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Jianqiang Xiang
Ningbo Medical Centre Lihuili Hospital
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Hong Li
Ningbo Medical Centre Lihuili Hospital

Corresponding Author:[email protected]

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Objectives: We investigated the molecular underpinnings of miR-205-3p in driving the proliferative behavior of endothelial cells originating from hepatocellular carcinoma (HCC). Methods: In our study, reverse transcription quantitative PCR analysis identified a significant upregulation of miR-205-3p in endothelial cells from HCC. This upregulation was consistently observed across various HCC cell lines and tissue samples. Further investigation highlighted the regulatory effect of miR-205-3p on the HINT1 gene. The interaction between miR-205-3p and the 3’ untranslated region of HINT1 was confirmed using a dual-luciferase reporter assay. Additionally, experiments involving MTT assays and flow cytometry demonstrated that lentivirus-mediated suppression of HINT1 enhanced cellular proliferation and reduced apoptotic activity in HCC cell models. Results: This study reveals that miR-205-3p contributes to the progression of endothelial cells from HCC by targeting and inhibiting HINT1, a tumor suppressor. Conclusions: This study provides a theoretical foundation suggesting that miR-205-3p may serve as a viable molecular target for gene therapy interventions in clinical settings.
25 Jan 2024Submitted to Cancer Reports
27 Jan 2024Assigned to Editor
27 Jan 2024Submission Checks Completed
27 Jan 2024Review(s) Completed, Editorial Evaluation Pending
01 Feb 2024Reviewer(s) Assigned