Graphene oxide (GO) is a widely used nanomaterial, whose unique
carbon-based structure bears abundant oxygen-containing functional
groups. An increasing number of investigations have been performed on
the effects of GO on plants due to its extensive use in various fields.
In this work, under the optimized GO concentration (6 mg/L), we found
that GO promoted the growth rate of the root of poplar. TEM images
depicted that the plasmolysis and an increased number of starch grains
were the two major physiological changes in the root cell samples. GO
adhered to the cell membrane on the inner side of the cell wall and
enhanced the toughness of the adventitious root. And the activity of
oxidoreductases were measured and SOD, POD were found to increase
significantly compared with the control group, but the increase of CAT
was not obvious. Related gene expression was enhanced at low
concentration of GO, but inhibited at high concentration. Our results
indicate the possible usage of GO as enhancers in the growth of poplar
tissue culture seedlings.