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Characterization and genome functional analysis of the psychrotrophic lignin-degrading bacterium Arthrobacter sp. C2 and the role of DyP in catalyzing the degradation of lignin
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  • Cheng Jiang,
  • Xiaohui Shen,
  • Yi Cheng,
  • Yuting Zhang,
  • Yue Wang,
  • Shanshan Sun,
  • Hanyi Jiang,
  • Hailian Zang,
  • Xinyue Zhao,
  • Ning Hou,
  • Ziwei Li,
  • Liwen Wang,
  • Hanjun Wang,
  • Chunyan Li
Cheng Jiang
Northeast Agricultural University
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Xiaohui Shen
Yichun University
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Yi Cheng
China Agricultural University
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Yuting Zhang
Northeast Agricultural University
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Yue Wang
Northeast Agricultural University
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Shanshan Sun
Northeast Agricultural University
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Hanyi Jiang
Northeast Agricultural University
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Hailian Zang
Northeast Agricultural University
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Xinyue Zhao
Northeast Agricultural University
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Ning Hou
Northeast Agricultural University
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Ziwei Li
Northeast Agricultural University
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Liwen Wang
Northeast Agricultural University
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Hanjun Wang
Northeast Agricultural University
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Chunyan Li
Northeast Agricultural University

Corresponding Author:[email protected]

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Abstract

In the cold regions of China, much of the lignin-rich corn straw accumulated due to low temperature limiting. However, the lack of psychrotrophic lignin-degrading strain resources hinders the lignin decomposition. Here, the whole genome of the psychrotrophic lignin-degrading bacterium Arthrobacter sp. C2, isolated in author’s previous work, was sequenced. Comparative genomics revealed that C2 contained unique genes related to lignin degradation and low-temperature adaptability. DyP may participate in lignin degradation and may be a cold-adapted enzyme. Moreover, DyP was proven to catalyze lignin Cα-Cβ bond cleavage. DyP gene deletion and complementation verified its ability to catalyze the first-step reaction of lignin degradation. Comparative transcriptome analysis revealed that the transcriptional expression of DyP gene was induced upregulation, and the genetic compensation mechanism endowed C2ΔDyP viability on lignin. This study improved the understanding of metabolic mechanism of psychrotrophic lignin-degrading bacteria and provided potential application options for energy-saving production using cold-adapted lignin-degrading enzymes.