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Integrated cyto-physiological and proteomic analyses reveal new insight into CMS mechanism in a novel upland cotton CMS line LD6A
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  • Ullah Najeeb,
  • Jie Zheng,
  • Aziz Khan,
  • Zhou Bujin,
  • Zhou Qiong,
  • Kong Xiangjun,
  • Liu Yiding,
  • Fang Liu,
  • Zhou Ruiyang
Ullah Najeeb
The University of Queensland School of Agriculture and Food Sciences

Corresponding Author:[email protected]

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Jie Zheng
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Aziz Khan
Guangxi University Guangxi Agriculture College
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Zhou Bujin
Guangxi University Guangxi Agriculture College
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Zhou Qiong
Guangxi University Guangxi Agriculture College
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Kong Xiangjun
Guangxi University Guangxi Agriculture College
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Liu Yiding
Guangxi University Guangxi Agriculture College
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Fang Liu
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Zhou Ruiyang
Guangxi University Guangxi Agriculture College
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Abstract

Cytoplasmic male sterile (CMS) system has extensively been used for exploiting hybrid vigor in plant breeding programs. However, poor understanding of molecular mechanism of fertility restoration in CMS is still a major constraint for its application in many crops. Using advanced analytical approaches, we elucidated the molecular pathways regulating CMS induction and fertility restoration in cotton. Reproductive structures of a novel CMS (LD6A) and its maintainer (LD6B) line were analyzed physiological and proteomic responses. Significant differential expression of proteins, such as Abrin, malate dehydrogenase, malic enzyme, isocitrate dehydrogenase, histone acetyltransferase was observed in novel CMS and its maintainer line. Transmission electron micrographs of mitochondrial structure in anther tapetum showed that the inner ridge of mitochondria in CMS line was relatively indistinct than that of LD6B with narrower membranous space at the tetrad stage. Further, relatively higher reactive oxygen species were accumulated in the anther of CMS than its maintainer line at pollen mother cell and tetrad stage. We suggest that abnormal sequence of mitochondrial ribosome gene rps4 and rpl10 and high expression of ribosome-inactivating protein gene Abrin in CMS line damaged mitochondrial membrane and consequently induced pollen sterility. These data provide new insight into CMS mechanism in cotton crops and a tool to develop new CMS germplasm resources.