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Drug resistance analysis of APEC carrying mcr-1 in some coastal areas of China from 2019 to 2020
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  • Wei Zhang,
  • Zimeng Hu,
  • Xinyi Chen,
  • Zhuohao Wang,
  • Genglin Guo,
  • Zhengjun Xu,
  • Qingan Zhou,
  • Xiankai Wei,
  • Yuqing Liu
Wei Zhang
Nanjing Agricultural University College of Veterinary Medicine

Corresponding Author:[email protected]

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Zimeng Hu
Nanjing Agricultural University College of Veterinary Medicine
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Xinyi Chen
Nanjing Agricultural University College of Veterinary Medicine
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Zhuohao Wang
Nanjing Agricultural University College of Veterinary Medicine
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Genglin Guo
Nanjing Agricultural University College of Veterinary Medicine
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Zhengjun Xu
Jiangsu Animal Disease Prevention and Control Center Nanjing 210000 China
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Qingan Zhou
Guangxi Animal Disease Prevention and Control Center Nan’ning 530000 China
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Xiankai Wei
Guangxi Animal Disease Prevention and Control Center Nan’ning 530000 China
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Yuqing Liu
Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Shandong Province Jinan 250100 China
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Abstract

Polymyxin is considered as one of the “last line of defense” for the treatment of multi-drug resistant bacteria. With the ever-increasing use of polymyxin in recent years, posing a serious risk to public health. In this study, 24 mcr-1-positive APEC isolates were detected from 493 APEC isolates which were isolated and identified from dead and sick poultry in China, and 57 isolates of APEC were selected for whole genome sequencing. The polymyxin MIC of 24 isolates of mcr-1-positive APEC was determined, and the results showed they were all resistant to polymyxin. Besides, 14 isolates of mcr-1-positive APEC plasmids were successfully conjugated to EC600. The whole genome sequencing results showed that the plasmid types related to the spread of mcr-1 gene were IncHI2 (15/24), IncX4 (10/ 24), IncI2 (4/24); two ST types ST93 (9/24), ST349 (4/24) and two serotypes O166:H15 (5/24), O21:H52 (7/24) accounted for a large proportion of APEC sequencing, showing that the isolated APEC have the diversity of ST types and serotypes. Besides, it is worth noting that 2 isolates of APEC resistant to meropenem were found, and they were detected to contain bla NDM-1 and bla NDM-4 genes, respectively. In this study, the epidemiological investigation of the mcr-1 gene was carried out on APEC in some coastal areas of China from 2019 to2020, and the results enriched the transmission data of the APEC isolates carrying the mcr-1 gene in waterfowl.