Multidimensional gene regulatory landscape of motor organ pulvinus in
the model legume Medicago truncatula
Nyctinastic leaf movement of Fabaceae is driven by the tiny motor organ
pulvinus located at the base of leaf or leaflet. Despite the increased
understanding of the essential role of ELP1 orthologs in determining
organ identity, key regulatory components and molecular mechanisms
underlying this movement remain largely unclear. Here, we used WT
pulvinus and the equivalent tissue in elp1 mutant to carry out
transcriptome and proteome experiments, and the omics data indicated
that there are multiple cell biological processes altered at gene
expression and protein abundance level during the pulvinus development.
In addition, comparative analysis of organ specific transcriptome from
different leaf tissues provided clues to illuminate the origin of
pulvinus and signaling pathways of leaf movement. Furthermore, mutants
of clock gene MtPRR5 confirmed its function in the leaf movement,
meanwhile auxin signal played in important role in pulvinus development.
By the way, molecular and histochemical results confirmed lots of cell
wall and photosynthesis related genes are involved in the pulvinus
development. This study provides a comprehensive insight of the
nyctinastic movement, further supplies a rich dataset to facilitate the
identification of novel players involved in nyctinastic movement.