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Influence of Colonies' Morphological Cues on Cellular Uptake Capacity of Amino Modified Nanoparticles
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  • Siyuan Huang,
  • Yingjun Yang,
  • Xiaoqiang Hou,
  • Jingyi Chen,
  • Guanjian Nie,
  • Bingshe Xu,
  • Shukai Ding
Siyuan Huang
Shaanxi University of Science and Technology
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Yingjun Yang
Shaanxi University of Science and Technology

Corresponding Author:[email protected]

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Xiaoqiang Hou
Shaanxi University of Science and Technology
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Jingyi Chen
Shaanxi University of Science and Technology
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Guanjian Nie
Shaanxi University of Science and Technology
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Bingshe Xu
Shaanxi University of Science and Technology
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Shukai Ding
Shaanxi University of Science and Technology
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Abstract

Micropatterning techniques were widely applied to manipulate morphology of colony and to reveal the critical role of spatial factors in tumorigenesis and gastrulation. However, the effect of morphological cues on bioactive substances treatment during these biological processes was still not clear. Therefore, the influence of colony's morphology on cellular uptake capacity of insoluble bioactive substance should be another essential factor in biomolecular induced cellular behaviors' variation. In this study, PDMS stencils were applied to control colonies' size, and relationship between cellular uptake capacity of nanoparticles and morphological cues of colonies was analyzed. Consequently, benefited from more nanoparticles within micropatterns, the larger colonies endocytosed more nanoparticles. Additionally, concentrated cells with high cell seeding density or located at the peripheral region of micropatterned colonies have higher cellular uptake capacity. The improved cellular uptake capacity at the peripheral region was mainly caused by the uneven distribution of nanoparticles. In addition, the high cell density promoted cellular uptake was benefited from the cortical actin accelerated clathrin-mediated endocytosis in circular-shaped cells.