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Lipid emulsion exerts a protective effect on vital organs by LPS/TLR4 pathway in acute organophosphate poisoning
  • haiyan hu,
  • gang Li
haiyan hu
hang zhou shi wei sheng ju
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gang Li
Zhejiang Provincial People's Hospital

Corresponding Author:[email protected]

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Abstract

Background:Organophosphorus poisoning is one of the common types of pesticide poisoning, which causes severe damage to vital organs. Reportedly, lipid emulsion combined with Pralidoxime significantly improves the prognosis of poisoning, but the mechanism is yet unclear;Purpose:It confirmed Lipid emulsion protect effect via LPS/TLR4 pathway on vital organs in acute organophosphorus poisoning;Methods:an organophosphorus poisoning rat model was established, and normal experimental methods were adopted. Hematoxylin-eosin (HE) observed tissue pathology, flow cytometry detected TLR4 expression, Enzyme-linked immuno sorbent assay(ELISA) detected inflammatory cytokines, and Reverse transcription PCR( RT-PCR) detected the mRNA expression of inflammatory cytokines. The role of LPS/TLR4 pathway in lipid emulsion treatment of organophosphorus poisoning was further verified by anti-TLR4. The results :It showed that after 24 h of survival, multiple organs were damaged in rats with organophosphorus poisoning. After treatment with Pralidoxime or lipid emulsion, the poisoning symptoms were slightly alleviated, but after treatment with Pralidoxime combined with lipid emulsion, the symptoms were significantly alleviated, and the expression level of TLR4 was significantly decreased in the MLT+Lipid group. After anti-TLR4 was used, the LPS/TLR4 pathway was blocked. Compared with the blocking group and the non-blocking group, the liver function and Cholinesterase(CHE) of the rats were significantly improved, the pathology of the lungs and heart were improved, and the inflammatory cytokines were significantly reduced, the symptoms of organ poisoning were relieved;Conclusion: Lipid emulsion is involved in the protective effect via LPS/TLR4 pathway on vital organs in acute organophosphorus poisoning.