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New insights on the role of HCN in root hair elongation through single cell proteomics.
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  • Lucia Arenas-Alfonseca,
  • Masashi Yamada,
  • Luis Romero,
  • Irene García
Lucia Arenas-Alfonseca
Instituto de Bioquimica Vegetal y Fotosintesis
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Masashi Yamada
Agricultural Biotechnology Research Centre Academia Sinica
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Luis Romero
Instituto de Bioquimica Vegetal y Fotosintesis
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Irene García
Instituto de Bioquimica Vegetal y Fotosintesis

Corresponding Author:[email protected]

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Abstract

Root hairs are specialized structures involved in water and nutrient uptake by plants. They elongate from epidermal cells following a complex developmental program. ß-cyanoalanine synthase (CAS), which is mainly involved in hydrogen cyanide (HCN) detoxification in Arabidopsis thaliana, plays a role in root hair elongation, as evidenced by the fact that cas-c1 mutants show a severe defect in root hair shape. In addition to root hairs, CAS C1 is expressed in the quiescent center and meristem. However, the cas-c1 mutation has no visible effect on either tissue, in both control and nutrient-deprivation conditions. To identify its role in root hair formation, we conducted single cell proteomics analysis by isolating root hair cells using Fluorescence-Activated Cell Sorting (FACS) from wild type and cas-c1 mutants. We also analyzed the presence of S-cyanylation, a protein post-translational modification (PTM) mediated by HCN and affecting cysteine residues and protein activity, in proteins of wild type and cas-c1 mutants. We found that several proteins involved in root hair development, related to the receptor kinase FERONIA signaling and to DNA methylation, are modified by this new post-translational modification.