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Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
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  • Adelaide Braga,
  • Daniela Gomes,
  • Joao Rainha,
  • Beatriz Cardoso,
  • Claudia Amorim,
  • Sara Silvério,
  • Maria Lobato,
  • Joana Rodrigues,
  • Ligia Rodrigues
Adelaide Braga
Universidade do Minho
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Daniela Gomes
Universidade do Minho
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Joao Rainha
Universidade do Minho
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Beatriz Cardoso
Universidade do Minho
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Claudia Amorim
Universidade do Minho
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Sara Silvério
Universidade do Minho
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Maria Lobato
CSIC
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Joana Rodrigues
Universidade do Minho
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Ligia Rodrigues
Universidade do Minho

Corresponding Author:[email protected]

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Abstract

Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB, that was able to produce 73.4 ± 1.6 g L−1 of FOS, with a productivity of 1.53 ± 0.03 g L-1 h-1 and a yield of 0.31 ± 0.03 gFOS gsucrose-1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process.