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Overcharged lipid metabolism in mechanisms of antitumor by Tremella Fuciformis Polysaccharide
  • Xiumin Li,
  • Qiaoling Su,
  • Yutian Pan
Xiumin Li
The Engineering Technological Center of Mushroom Industry

Corresponding Author:[email protected]

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Qiaoling Su
The Engineering Technological Center of Mushroom Industry
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Yutian Pan
the Engineering Technological Center of Mushroom Industry
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Abstract

Tremella Fuciformis polysaccharides (TFP) is a natural macromolecular compound well-known for whitening, lipid and immunity regulation. To evaluate the effect of TFP on melanogenesis, lipid metabolism programmes and immune system of B16 cells in vitro and in vivo, the cell proliferation, cell cycle and apoptosis were analyzed; B16 cells treated with TFP were inoculated subcutaneously to explore the effect of TFP on B16 cells in vivo. WB, immunohistochemistry, and immunofluorescence staining and transcription analysis of tumor were utilized to assess the expression of key molecules in melanin process, lipid metabolism and immunity. We found that TFP promoted the B16 apoptosis, and induced G2/M cell cycle arrest, which was associated with cell cycle pathways enrichment. As expected, TFP blocked melanogenesis in vivo and decrease Tyr mRNA and protein in vitro, and increase melanosome transport related mRNA in vivo. TFP induced the GLUT4 and CD36 with the resulting increase in the uptake of lipids, which suppressed SREBP1, p-AMPK drastically, increased lipid in cell membrane, ER and nucleus; as well as raise lipid metabolism related RNA-seq prolife in vivo. More lipid binding, upregulated lipid storage, and elevated triglyceride and lipid catabolism resulted in disruption of cell volume homeostasis and activated innate immune response, thus inhibited melanoma development and progression. These data reveals a novel molecular mechanism involved in TFP antitumor effect, through lipid metabolism.