The plant bug, Pachypeltis micranthus Mu et Liu (Hemiptera:
Miridae), is a potential biological control agent for Mikania
micrantha H.B.K. (Asteraceae; one of the most invasive weeds
worldwide). To date, only a few studies have investigated plant bugs.
Here, we performed a chromosome-level genome assembly of P.
micranthus using MGISEQ-2000 short-read, Nanopore, PacBio long-read,
and high-throughput chromosome conformation capture (Hi-C) techniques.
The assembled genome was 712.72 Mb in size, with a contig N50 of 16.84
Mb. Using the Hi-C technique, 71 scaffolds were assembled into 15
chromosomes, accounting for 99.96%. We predicted 11,746 protein-coding
genes in P. micranthus with 96.20% complete benchmarking
universal single-copy orthologs. Phylogenomic analysis showed that
P. micranthus and two other Miridae bugs (Apolygus lucorum
and Nesidiocoris tenuis) diverged from the common ancestor
approximately 200.01 million years ago. Chromosome synteny analysis
between P. micranthus and A. lucorum indicated high-level
synteny. Many gene families including chemosensory genes and digestive
and detoxification enzyme genes—were significantly expanded in the
P. micranthus genome. These expanded gene families may indicate
the bug to adapt to the single host plant. This high-quality
chromosome-level genome assembly provides an invaluable resource for
further molecular and evolutionary research on mirid bugs and also
provides a basis for further research on biological control mechanisms
for M. micrantha.