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Human umbilical cord mesenchymal stem cells can efficiently improve neurobehavioral status and alleviated brain injury in hypoxia/ischemia-induced cerebral palsy rat model via down-regulating the NogoA/NgR/Rho pathway
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  • Yaoling Yaoling Luo,
  • Zhengyi He,
  • Minhong Zhang,
  • Zhengwei Zou,
  • Lincai Li,
  • Jiayang Qu,
  • Qianqian Xu,
  • Junsong Junsong Ye
Yaoling Yaoling Luo
Gannan Medical University
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Zhengyi He
Gannan Medical University
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Minhong Zhang
Gannan Medical University
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Zhengwei Zou
Gannan Medical University
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Lincai Li
Gannan Medical University
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Jiayang Qu
Gannan Medical University
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Qianqian Xu
Gannan Medical University
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Junsong Junsong Ye
Gannan Medical University

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Abstract

Abstract: Background: At present, there is no ideal treatment available for CP patients and most of them will still suffer adverse outcomes. NogoA/NgR/Rho pathway is very important to the nerve growth, CP injury is inevitably accompanied by the regeneration and repair of neurons and axons. Purpose: In this study, we might clarify the NogoA/NgR/Rho pathway functional role in mediating HUCMSCs to improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced CP rat model. Methods: The CP rat model was established by ligating the left common carotid artery and anoxia for 2.5 h, and HUCMSCs were intravenous injected to the modeled rats. Results: Compared with CP+PBS group and CP group rats, HUCMSCs transplantation can significantly improve the neurobehavioral situation, attenuated brain pathological injury, inhibit apoptosis of brain nerve cells and the activation of astrocytes in CP rats. The expression of NogoA、NgR、RhoA relative mRNA and protein in brain tissues of rats in the CP+PBS group and CP group rats were significantly lower than those of in the sham+PBS and CP+HUCMSCs group. The expression of Rac-1、Cdc42 relative mRNA and protein in brain tissues of rats in the sham and CP+HUCMSCs group was significantly higher than those of in CP+PBS group and CP group rats. Conclusion: This study confirmed that HUCMSCs can efficiently improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced cerebral palsy rat model via down-regulating the NogoA/NgR/Rho pathway.