Anti-inflammatory screening biomarkers on an LPS-induced RAW 264.7 cell
model: a Systematic Review and Meta-analysis.
The inflammation is a response to some injury aimed at reestablishing
homeostasis of the affected tissue. The in vitro model using RAW 264.7
cells has been widely used, as experimental model to assess the
inflammatory response. However, there is still no consensus on which
inflammatory mediators should initially be measured to screen for
possible anti-inflammatory effects. To determine the rationality of
measuring different inflammatory mediators together with NO, we carried
out this systematic review and meta-analysis. A total of 17 studies were
included, and LPS-induced cells produced high NO levels compared to
non-LPS induced, and this production was not related to cell density.
TNF-α, IL-1β, and IL-6, also showed high levels after cells had been
stimulated with LPS; however, these effects were related to cell
density. Finally, was found that measurement of NO levels alone may be
sufficient to screen for possible anti-inflammatory action in the
context of this model.