loading page

Poly(I:C) and CpG Improve the Assembly of Foot-and-mouth Disease Virus-like Particles and Immune Response in Mice
  • +5
  • Haiyun Liu,
  • Zhidong Teng,
  • hu dong,
  • jiajun li,
  • yun zhang,
  • manyuan bai,
  • Shiqi Sun,
  • huichen guo
Haiyun Liu
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile
Zhidong Teng
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile
hu dong
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile
jiajun li
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile
yun zhang
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile
manyuan bai
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile
Shiqi Sun
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute

Corresponding Author:[email protected]

Author Profile
huichen guo
Chinese Academy of Agricultural Sciences Lanzhou Veterinary Research Institute
Author Profile

Abstract

Virus-like particles(VLPs) represent a safe and effective vaccine platform, improving assembly efficiency is necessary to reduce the cost of vaccine production, and elicit a strong immune response. In this study, CpG and Poly(I:C) were selected as scaffolds to facilitate the assembly of FMDV VLPs in vitro. The particle size, surface potential, gel retardation assay, nuclease digestion experiments, size-exclusion chromatography, transmission electron microscopy and circular dichroism analysis showed that CpG and Poly(I:C) were successfully encapsulated inside of VLPs, and have no effect on shape and sizes. The peak area of 75S of co-assembled CpG-VLPs and Poly(I:C)-VLPs were significantly increased than FMDV-VLPs(P<0.0001). With increasing the assembly efficiency of CpG-VLPs and Poly(I:C)-VLPs, higher neutralizing antibodies were elicited. Although, CpG or Poly(I:C) as adjuvant enhanced the proliferation level of spleen lymphocytes, but the proportion of CD4+ T cells and CD8+ T cells in the lymphocyte were significantly increased after stimulating by co-assembled CpG-VLPs (P<0.01) and Poly(I:C)-VLPs (P<0.001). This study demonstrated that CpG and Poly(I:C) not only improved the assembly efficiency of FMDV VLPs, also had an adjuvant activity to enhance the immune response, Furtherly, proposes a new idea and optimized strategy for improving the assembly efficiency of VLPs and contributes to the development of VLPs vaccine.