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Diagnostic Value of exosome miRNA combined with RT3D-STE in STEMI and NSTEMI
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  • Xiaozhu Chen,
  • Fengrong Huang,
  • Yunhong Liu,
  • Shujun Liu,
  • Gangwen Tan
Xiaozhu Chen

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Fengrong Huang
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Yunhong Liu
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Shujun Liu
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Gangwen Tan
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Abstract

BACKGROUND MicroRNAs (miRNAs) play essential roles in acute myocardial infarction (AMI), but little is known regarding the value of exosome miRNA combined with real-time three-dimensional spot tracking echocardiography (RT3D-STE) between ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI). AIM To analyze the correlation between exosomal miRNA and strain parameters of RT3D-STE. METHODS The present study collected plasma samples from thirty-four patients with AMI, and employed high-throughput sequence technology and Real-time Quantitative Polymerase Chain Reaction (qRT-PCR) to identify the differentially expressed miRNAs. The pearson correlation coefficient is used to measure the strength of a linear association between differ-expressed miRNAs and strain parameters of RT3D-STE. RESULTS Twenty-eight difference expressed plasma exosomes miRNAs were identified in STEMI Compared with NSTEM. Among them, there are ten miRNAs (miR-152-5p, miR-3681-5p, miR-193a-5p, miR-193b-5p miR-345-5p, miR-125a-5p, miR-365a-3p, miR-4520-2-3p, hsa-miR-193b-3p and hsa-miR-5579-5p) with a Pearson correlation greater than 0.6 with RT3D-STE strain parameters. Target genes of these ten miRNAs are analyzed for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment, and they were found to be mainly involved in the cellular metabolism processes and HIF-1 signaling pathway. CONCLUSION These ten xosomal miRNA combined with RT3D-STE may have important value in STEMI and NSTEMI.