long non-coding RNA OIP5-AS1 suppresses microRNA-92a to augment
proliferation and metastasis of ovarian cancer cells through
Objective: Recently, long non-coding RNAs (lncRNAs) and microRNAs
(miRNAs) have been identified as essential biomarkers during development
of malignancies. This study was performed to study the roles of lncRNA
opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) and miR-92a
in ovarian cancer (OC). Methods: OIP5-AS1, miR-92a and integrin alpha 6
(ITGA6) expression in OC tissues and cells was assessed. The screened OC
cells were respectively with OIP5-AS1 silenced vector, miR-92a
mimic/inhibitor or depleted or restored integrin alpha 6 (ITGA6) vector.
The viability, migration, invasion and apoptosis of the cells were
determined and the levels of epithelial-mesenchymal transition
(EMT)-related proteins were also measured. The interactions between
OIP5-AS1 and miR-92a, and between miR-92a and ITGA6 were confirmed.
Results: OIP5-AS1 and ITGA6 were upregulated while miR-92a was
downregulated in OC. MiR-92a was a target of OIP5-AS1 and ITGA6 was
targeted by miR-92a. Inhibited OIP5-AS1 or ITGA6 or elevated miR-92a
repressed EMT, viability, migration and invasion, and promoted apoptosis
of OC cells. These effects that induced by silenced OIP5-AS1 could be
reversed by miR-92a inhibition while those that induced by up-regulated
miR-92a were reduced by restored ITGA6. Conclusion: OIP5-AS1 silencing
promoted miR-92a to repress proliferation and metastasis of OC cells
through inhibiting ITGA6.