Ultrasound is a non-invasive technique that has been shown to affect the
cell proliferation, migration, differentiation, and promote the
regeneration of cell. Our previous studies had proved that ultrasound
improve natural killer (NK) cells mediated ovarian cancer therapy. To
further explore the underlying mechanisms of ultrasound in NK cell and
ovarian cancer cell, both cells treated by low-intensity pulsed
ultrasound (LIPUS) and detected through ELISA analysis and western blot.
The result demonstrated that the expression of Granzyme A, Granzyme B,
perforin protein secreted by NK cells were increase. In the NK-SKOV3
co-culture system, the expression level of membrane permeability related
proteins in the Wnt/β-catenin pathway of SKOV3 cells increased. This
study presented cellular regulation in NK cells and ovarian cancer cells
after LIPUS treatment and may expand the understanding of the complex
molecular mechanism of LIPUS treatment on NK cells.