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A traditional clinical prescription, Langchuangding, induces β-oestradiol-activated Jurkat T cell apoptosis to treat systemic lupus erythaematosus
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  • Huiqing Lv,
  • Qianqian Li,
  • Zhijun Xie,
  • Haichang Li,
  • Weijie Wang,
  • Huanpeng Gu,
  • Chengping Wen
Huiqing Lv
Zhejiang Chinese Medical University

Corresponding Author:[email protected]

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Qianqian Li
Zhejiang Chinese Medical University
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Zhijun Xie
Zhejiang Chinese Medical University
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Haichang Li
Zhejiang Chinese Medical University
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Weijie Wang
Zhejiang Chinese Medical University
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Huanpeng Gu
Zhejiang Chinese Medical University
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Chengping Wen
Zhejiang Chinese Medical University
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Abstract

Systemic lupus erythaematosus (SLE) is a chronic autoimmune disease associated with inflammation and organ damage. Based on accumulating evidence, activated T cells are key cells promoting the pathogenesis of SLE. Langchuangding (LCD) is an effective clinical traditional Chinese medicine prescription for treating SLE with few side effects and good patient compliance. LCD has proven great potential as a supplement to conventional treatments in previous clinical studies However, few studies have investigated its potential mechanism. Here, we prepared LCD-treated rat serum (LCDs) and evaluated their effects on activated Jurkat T cells stimulated with β-oestradiol in vitro. We developed a sensitive and reliable high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) method for the quality control of LCDs. T cell proliferation and apoptosis were detected with the CCK-8 assay and flow cytometry analysis. Caspase-3 mRNA and protein expression were detected using real-time PCR and western blotting, respectively. Two compounds, ferulic acid and isoferulic acid were simultaneously identify and their concentrations of high, middle and low doses of LCDs (H, M and L) were determined respectively. LCDs treatment inhibited the proliferation (P<0.05) of activated Jurkat T cells and induced apoptosis by up-regulating the mRNA and protein levels of caspase-3. Taken together, our results suggested that LCDs treatment depressed β-oestradiol-activated T cell proliferation by inducing apoptosis through caspase-3 activation, finally contributing to the amelioration of SLE. Thus, LCD potentially represents a promising therapeutic prescription for SLE supplement treatment with no adverse effects.