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Liraglutide Attenuates Gentamicin-Induced Nephrotoxicity in Rats by Reducing Oxidative Stress
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  • Recep Burak Degirmentepe,
  • Muammer Bozkurt,
  • OSMAN CAN,
  • mustafa erkoc,
  • hazal ozturk gurgen,
  • funda yildirim,
  • fatih hunc,
  • fatma ceyla eraldemir,
  • Alper Otunctemur
Recep Burak Degirmentepe
Sakarya Universitesi

Corresponding Author:[email protected]

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Muammer Bozkurt
Basaksehir Cam and Sakura City Hospital
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OSMAN CAN
Basaksehir Cam and Sakura City Hospital
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mustafa erkoc
Okmeydani Training and Research Hospital
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hazal ozturk gurgen
İstanbul Üniversitesi-Cerrahpaşa
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funda yildirim
İstanbul Üniversitesi-Cerrahpaşa
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fatih hunc
Kocaeli Universitesi
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fatma ceyla eraldemir
Kocaeli Universitesi
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Alper Otunctemur
okmeydani training and research hospital
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Abstract

Purpose Nephrotoxicity is a major complication of gentamicin (GEN), which is widely used in the treatment of severe Gram-negative infections. As we know, treatment with liraglutide has been shown to reduce oxidative stress. Therefore, we evaluated the potential protective effect of liraglutide against GEN-induced nephrotoxicity in rats. Methods Twenty-eight rats were randomly divided into four groups: control group (Group 1); rats intraperitoneally injected with GEN (100 mg/kg/day; Group 2); rats treated with GEN plus distilled water (Group 3); and rats treated with GEN plus liraglutide (0.6 mg/kg/day; Group 4). After 15 days, the rats were sacrificed, their kidneys taken, and blood analysis performed. Tubular necrosis, interstitial fibrosis and inducible nitric oxide synthetase (iNOS) scores were determined histopathologically in a part of kidneys; malondialdehyde (MDA), reduced glutathione (GSH), E-cadherin and transforming growth factor β1 (TGF-β1) levels were determined in other part of kidneys. Results The GSH levels in renal tissue of only GEN-treated rats were significantly lower than others, and administration of liraglutide to rats significantly increased the level of GSH. The group that was given GEN plus liraglutide had significantly lower MDA, TGF - β1 and E - cadherin levels than that given GEN alone. The rats treated with GEN+liraglutide indicated a less severe tubular necrosis, and their glomeruli maintained a better morphology compared to GEN group. iNOS expression was higher in liraglutide administrated group than the group that was applied only GEN. Conclusion Liraglutide exerts protective effects on GEN-induced kidney damage by reducing oxidative stress in rat model.