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Tacheng tick virus 1 and Songling virus infection in great gerbils ( Rhombomys opimus ) in border regions, northwestern China
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  • Yuanzhi Wang,
  • Na Ji,
  • Gang Liu,
  • Shiyi Wang,
  • Shanshan Zhao,
  • Zhiqiang Liu,
  • Wenbo Tan,
  • Nan Wang,
  • Fengshi Li
Yuanzhi Wang
Shihezi University School of Medicine

Corresponding Author:[email protected]

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Na Ji
Shihezi University School of Medicine
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Gang Liu
Shihezi University School of Medicine
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Shiyi Wang
Shihezi University School of Medicine
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Shanshan Zhao
Shihezi University School of Medicine
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Zhiqiang Liu
Xinjiang Academy of Animal Science
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Wenbo Tan
Shihezi University School of Medicine
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Nan Wang
Shihezi University School of Medicine
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Fengshi Li
Shihezi University School of Medicine
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Abstract

Tacheng tick virus 1 (TcTV-1) was firstly found in a tick bitten patient in Qinghe County (border county of China-Mongolia), Xinjiang Uygur Autonomous Region (XUAR), and further detected in pastured cattle and sheep. Songling virus (SGLV) was isolated in tick-borne patients in Heilongjiang Province (border region of China-Russia) and Inner Mongolia Autonomous Region (border region of China-Mongolia). The aim of this study was to detect TcTV-1, SGLV and Tamdy virus (TAMV, first found in a tick bitten patient from Kyrgyzstan) in great gerbil. A total of 276 great gerbils were collected in Manas County (common county) and Altaw City (border city of China-Kazakhstan), XUAR, northwestern China. The total RNA of individual spleen tissue was extracted, and the viral L segments of TcTV-1, SGLV and TMAV were detected by transcriptase-polymerase chain reaction. The amplification products were cloned and sequenced, and phylogenetic analyses were conducted by MEGA7.0. The results indicated that 2.9% (8/276) and 2.2% (6/276) of spleens were respectively tested positive to the viral L segments for TcTV-1 and SGLV, and TAMV was not detected in these samples. SGLV in great gerbil shared 93.7% (236/252 nt) and 94.0% (78/83 aa) identities to that of SGLV (MT328776) detected in patients in northeastern China. TcTV-1 in great gerbil was the closest to that of TcTV-1 from a patient in China (MK554672) with 98.5% (797/809 nt) and 98.9% (265/268 aa) identities. This is the first molecular evidence for the presence of TcTV-1 and SGLV in great gerbil. SGLV presented highly genetic diversity correlated with their geographical location. Tamdy orthonairovirus surveillance in multiple border regions of China, Kazakhstan, Kyrgyzstan, Russia and Mongolia in more wild animals is necessary.