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Whole genome resequencing infers genomic basis of the Giant phenotype in Betta splendens
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  • Le Wang,
  • Fei Sun,
  • May Lee,
  • Gen Yue
Le Wang
Temasek Life Sciences Laboratory
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May Lee
Temasek Life Sciences Laboratory Ltd
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Gen Yue
Temasek Life Sciences Laboratory

Corresponding Author:[email protected]

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Understanding the genetic basis of phenotypes is of importance in evolutionary biology, and genetic improvement of economic animals. The Giant strain of fighting fish, Betta splendens, provides a unique material for dissecting the genetic architecture of overgrowth of body size because of its giant body size. We re-sequenced and analyzed the genomes of 54 fighting fish. Genome-wide FST and selective sweeps scans using 3,582,429 DNA variants revealed that three genomic regions at chr1, chr9 and chr11 were associated with the giant body size. These regions, with a total length of ~ 3.5 Mb, were highly diverged between the Giant and the non-Giant bettas. In contrast, no signature of selection was detected in the wild type fish. Transcriptome analysis in the brain and muscle samples from Giant and non-Giant betta fish identified 14 candidate genes that were likely responsible for the giant body size. Our data provide novel insight into the genetic basis of body size variation. The genome and transcriptome sequences, DNA sequence variants and candidate genes for body size provide valuable resources for further biological and evolutionary studies, as well as for rapid improvement of growth-related traits.