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Phytotoxic effect on chloroplast and UHPLC-HRMS based untargeted metabolomic responses in Allium tuberosum Rottler ex Sprengel exposed to antibiotics
  • +6
  • kiran khan,
  • Barkat Ali,
  • Shuang Zhang,
  • Peter Stoffella,
  • Yushi Shi,
  • Xia Qian,
  • Xiaoqain Cui,
  • Zeeshan Ali,
  • Ya Guo
kiran khan
Jiangnan University
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Barkat Ali
Jiangnan University
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Shuang Zhang
Jiangnan University
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Peter Stoffella
University of Florida
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Yushi Shi
Dalian chem data information ltd
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Xia Qian
Jiangnan University
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Xiaoqain Cui
Tianjin University
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Zeeshan Ali
National Agriculture Research Center, Pakistan
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Ya Guo
Jiangnan University

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Abstract

Introduction of antibiotics into agricultural fields via using reclaimed waste water or manure application causes toxic effects on environment and pose serious health risks to humans. This study investigated the uptake of antibiotics, their effect on metabolic pathways, and chloroplast structure of Allium tuberosum exposed to different antibiotics stresses. Disturbances in chloroplast structure and increase in number of mitochondria and plastoglobulli have been suggested defensive mechanism against antibiotic stress by extending variations in chlorophyll fluorescence variables resulted in alterations to metabolic activities. An untargeted metabolomic analysis was conducted to explore the comprehensive profiling of metabolites in response to the applied antibiotic stresses because metabolomic approach directly assesses the physiological status of plant. Simultaneously, metabolomic profiling demonstrated that NFL stress regulated more of its metabolic pathways than OTC and TC. Some of the metabolic pathways varied among the antibiotic treatments. Metabolic variations as a result of antibiotics stress highlighted pools of metabolites that affect the metabolic activities, chlorophyll fluorescence, and ultrastructural adjustments that stimulate defensive impact in A .tubersoum. These novel findings are useful in providing an insight of metabolic destabilization as well as metabolic changes in defensive mechanism and better understanding of stress response of A. tuberosum to different antibiotics.