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Genome-wide association study identifies variants of GhSAD1 conferring cold tolerance in cotton
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  • Chang Ge,
  • Li Wang,
  • Yong Yang,
  • Rui Liu,
  • Shi Li,
  • Shao Liu,
  • Jing Chen,
  • Qian Shen,
  • Hui Ma,
  • Yang Li,
  • Si Zhang,
  • Chao Pang
Chang Ge
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Li Wang
Zhengzhou University
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Yong Yang
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Rui Liu
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Shi Li
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Shao Liu
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Jing Chen
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Qian Shen
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Hui Ma
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Yang Li
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Si Zhang
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Chao Pang
Chinese Academy of Agricultural Sciences Cotton Research Institute

Corresponding Author:[email protected]

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Abstract

Cold stress is a major environmental factor affecting plant growth and development. Although some plants have developed resistance to cold stress, the molecular mechanism underlying it is poorly understood. Here, we identified cold tolerance gene in cotton (Gossypium hirsutum L.) using genome-wide association study (GWAS) with 200 cotton accessions collected from different regions, and identified variations of a short chain alcohol dehydrogenase gene GhSAD1 that response to cold stress. The virus induced gene silencing (VIGS) and Arabidopsis overexpression experiments showed that GhSAD1 roles in cold-stress response.GhSAD1HapB introduction considerably increased cold tolerance in Arabidopsis transgenic plants, whereas GhSAD1HapA plants exhibited cold-sensitive phenotype. GhSAD1HapB silencing plants exhibited a decrease of abscisic acid (ABA) content and overexpression GhSAD1HapB in Arabidopsis increased ABA content. Additionally, we found that GhSAD1HapB regulated cold stress responses through the activity of c-repeat binding factor, which binds to the promoter region of the gene and regulates ABA signalling pathway under cold conditions. Overexpression of GhSAD1HapB induced cold-regulated (COR) genes expression and improved anti-cold metabolites under cold stress. Collectively, the findings of the study contribute to our understanding of the underlying mechanism of GhSAD1 in cold-stress response in cotton. Moreover, GhSAD1 could serve as candidate gene in improving crop stress