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Optical properties of biochemical compositions of microalgae within spectral range from 300 to 1700 nm
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  • Xingcan Li,
  • Li Lin,
  • Minghui Wu,
  • Bowei Xie,
  • Jia-Yue Yang,
  • Junming Zhao
Xingcan Li
Northeast Electric Power University

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Li Lin
Shandong University
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Minghui Wu
Northeast Electric Power University
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Bowei Xie
Harbin Institute of Technology
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Jia-Yue Yang
Shandong University
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Junming Zhao
Harbin Institute of Technology
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Abstract

A combination method (DOPTM-EM) using both DOPTM (double optical pathlength transmission method) and EM (ellipsometry method) is used to acquire the optical constants of protein, lipid and carbohydrate of Haematococcus pluvialis, Nannochloropsis sp. and Spirulina in both solid and solution state within the visible and near-infrared spectral range. For different types of microalgae, the refractive index of protein and carbohydrate in solid state are similar to each other, but show an observed difference from lipid in solid state. The refractive index of protein and carbohydrate in solution state presents a visible distinction in the researched spectral range. The absorption index of protein, lipid and carbohydrate in solid state for these three types of microalgae are close to each other in the spectral range 300-500 nm. However, an observed difference is shown in the spectral range 500-1700 nm. For ease of application, the refractive index of biochemical composition of microalgae was fitted based on the Sellmeier equation.