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Premature Centromere Division (PCD) as a Cause of Genome Instability in Children with Acute Lymphoblastic Leukemia
  • Artur Sirenko
Artur Sirenko
Vasyl Stefanyk Precarpathian National University

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Abstract

Premature centromere division (PCD) and C-anaphase are well known phenomena in leukemias. However, their biological significance is not well understood. We describe the relationship of levels of PCD and C-anaphase with leukemic phase, blasts, aneuploidy and polyploidy in children with acute lymphoblastic leukemia (ALL). Methods Cytogenetic preparations of bone marrow (BM) and peripheral blood (PB) cells from 57 children with ALL and 15 healthy children were evaluated. For children with ALL, cells were examined prior to treatment in the acute phase and during remission. PCD and C-anaphase designations were assigned to chromosomal preparations and analyzed. Results The PCD and C-anaphase levels in BM and PB cells were significantly higher in the acute phase of ALL and decreased, approaching normal during remission. Strong correlations were found between PCD levels in PB (r = 0.890) and BM (r = 0.896) cells compared with blast levels in PB cells. Strong correlations were observed between PB levels of aneuploid (r = 0.832) and polyploid (r = 0.955) cells compared with PCD levels in PB cells. The C-anaphase levels in PB (r = 0.139) and BM (r = 0.171) compared with blasts in PB did not demonstrate a positive correlation. Conclusions Our data demonstrates that PCD is a cause of genome instability associated with ALL and that the phenomenon of PCD and C-anaphase provide additional criteria for diagnosis of ALL and its remission.