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DEPOLYMERIZATION OF LIGNIN BY EXTRACELLULAR ACTIVITY OF PYCNOPORUS CINNABARINUS, TO OBTAIN CELLULOSE
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  • Margarita M. Brambila,
  • Raymundo Guzmán-Gi,
  • Hugo Velasco-Bedrán,
  • Oscar González-Brambila
Margarita M. Brambila
Universidad Autónoma Metropolitana Azcapotzalco

Corresponding Author:[email protected]

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Raymundo Guzmán-Gi
Universidad Autónoma Metropolitana Azcapotzalco
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Hugo Velasco-Bedrán
Instituto Politécnico Nacional
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Oscar González-Brambila
Aeropuertos y Servicios Auxiliares
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Abstract

A biotechnological process for cellulose recovery from cactus waste through depolymerization of lignin by Pycnoporus cinnabarinus, is presented. The delignification is carried out by fermentation in a batch stirred bioreactor, with a culture medium enriched with nutrients and minerals without added carbon sources, during a six-day span under continuous feeding of oxygen. A factorial design of experiments (DOE) for eight sets of factor values was selected for this study. The factors were: particle size, pH level, and process temperature. For each experiment, biomass, total reducing carbohydrates, and dissolved oxygen concentrations were measured every 24 hours. At the end of each experiment, the percentage of delignification, and cellulose recovery was measured by Infrared (IR) spectroscopy. Up to 67% of delignification and 22% of cellulose recovery were obtained by the process. These results were analyzed by a factorial DOE in order to maximize each response individually and to optimize both responses together. The delignification of Opuntia ficus indica thorns has not been previously reported to our knowledge.