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Genome-wide association study identifies variants of GhSAD1 conferring chilling tolerance in cotton
  • +9
  • Chang Ge,
  • Li Wang,
  • Yong Yang,
  • Rui Liu,
  • Shi Li,
  • Shao Liu,
  • Jing Chen,
  • Qian Shen,
  • Hui Ma,
  • Yang Li,
  • Si Zhang,
  • Chao Pang
Chang Ge
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Li Wang
Zhengzhou University
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Yong Yang
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Rui Liu
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Shi Li
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Shao Liu
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Jing Chen
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Qian Shen
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Hui Ma
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Yang Li
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Si Zhang
Chinese Academy of Agricultural Sciences Cotton Research Institute
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Chao Pang
Chinese Academy of Agricultural Sciences Cotton Research Institute

Corresponding Author:[email protected]

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Abstract

Cold stress is a major environmental factor affecting plant growth and development. Although some plants have developed resistance to cold stress, the molecular mechanism underlying it is poorly understood. Therefore, the aim of this study was to identify genes conferring cold resistance in cotton. Here, we identified cold tolerance gene in cotton (Gossypium hirsutum L.) using genome-wide association study (GWAS) with 200 cotton accessions collected from different regions. A variant of the gene was also identified. The results of the study showed that GhSAD1HapB improved cold-stress resistance in cotton by increasing abscisic aldehyde (ABAld) and amino acid contents under chilling stress conditions. GhSAD1HapB introduction considerably increased chilling tolerance in Arabidopsis transgenic plants, whereas GhSAD1HapA plants exhibited chilling-sensitive phenotype. Additionally, we found that GhSAD1HapB regulated chilling stress responses in cotton through the activity of c-repeat binding factor, which binds to the promoter region of the gene and regulates the ABA signalling pathway under chilling conditions. Overexpression of GhSAD1HapB induced COR genes expression and improved metabolic profile under cold stress. Collectively, the findings of the study contribute to our understanding of the underlying mechanism of GhSAD1 in cold-stress response in cotton. Moreover, GhSAD1 could serve as candidate gene in improving crop stress.