Different Cervical--vaginal Microbiome and Metabolism Composition in a
Promising Study of Cervical Lesion
Objective To determine the relationship between local microenvironment
and cervical cancer (CC). Discovering differential florae and
metabolites associating with CC. Design Observational study.
Participants 10 LSIL patients, 10 HSIL patients, 10 CC patients and 10
healthy controls were enrolled in our study. Methods We performed 16S
rDNA sequencing and metabolomic analysis in the cervicovaginal fluid to
reveal the differential florae and metabolites during cervical
carcinogenesis. Results Carcinogenesis was associated with alterations
in microbiome diversity, individual taxa, and functions. Bacterial
diversity was increased and the composition was changed by the influence
of cervical cancer carcinogensis. Lactobacillus was significantly
reduced in LSIL and CC patients. On the contrary, the relative abundance
of Prevotella was significantly elevated in LSIL patients. Lipids were
significantly declined in LSIL and HSIL patients compared to HC
participants, and significantly elevated in CC compared to LSIL and HSIL
patients. Pathway enrichment analysis found phenylalanine, tyrosine and
tryptophan biosynthesis pathway was crucial in cervical carcinogenesis.
Conclusions These results showed that microbic and metabolomic profiling
are capable of distinguishing CC from precancer and highlighted
potential biomarkers for the early detection of cervical dysplasia.
These differential microorganisms and metabolites expected to become a
potential tool to assist in the diagnosis of cervical cancer. Funding We
would like to thank Development & Demonstration Program of Wuxi
(N20192004), Key Research & Development Program of Jiangsu Province
(BE2015617), The 5th Phase of “Project 333” of Jiangsu (BRA2019024)
and Innovation and Entrepreneurship Training Program for College
Students in Jiangsu Province (KYCX19_1182) for their support.