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High--quality RNA improves sensibility of colorimetric RT-LAMP technique to detect SARS-CoV-2
  • Marta Puigmulé
Marta Puigmulé
Institut de Recerca Biomèdica de Girona Dr. Josep Trueta

Corresponding Author:[email protected]

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The global SARS-CoV-2 pandemic has required a rapid and reliable diagnostic test. RT-qPCR is currently the gold standard method for standard SARS-CoV-2 detection. Here we developed a protocol based on RT-LAMP and we compare the results obtained in fresh RNA extracts and in RNA samples with some freezing/thawing cycles, with those obtained using the standard method. We demonstrated that RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct value between 0-35